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Bertil Persson 0000-0002-5502-5972 - ORCID Connecting

H2AX is a meiosis-specific isoform of histone H2A. Upon DSB formation, rapid accumulation of  Mar 5, 2021 H2AX (H2A.X Variant Histone) is a Protein Coding gene. Diseases associated with H2AX include Nijmegen Breakage Syndrome and Ataxia-  May 24, 2011 Phosphorylation of the minor histone H2Ax (γH2Ax) has been a (2008) Assessment of DNA double-strand breaks and gammaH2AX induced  Mar 11, 2020 The histone variant H2AX phosphorylated on serine 139, named γ-H2AX, is a canonical DNA double-strand breaks marker. During mammalian  Chromatin remodeling to prevent dissociation of break ends and enhance DSB processing and repair efficiency The 𝛾 H2AX foci help keeping the broken DNA   Purpose: To assess the use of phosphorylated histone H2AX protein (γ-H2AX) in human blood leukocytes as a rapid screening tool for radiation biodosimetry  Anti-Gamma H2AX (phospho-Ser139) antibody, Rabbit monoclonal (H2AX Ser139 p ); HEK 293 cells, monoclonal RM224, purified immunoglobulin; Suitable for  Feb 4, 2010 Phosphorylation of the Ser-139 residue of the histone variant H2AX, forming γH2AX, is an early cellular response to the induction of DNA  Oct 11, 2018 Gamma‐H2AX is used widely as a DNA damage marker in vitro, since the availability of very specific antibodies allows its detection and  The gamma-H2AX assay enables screening for functional integrity of DNA double strand break (DSB) repair, and a broad range of genetic and epigenetic  Oct 25, 2012 In addition to γ-H2AX staining in specific locations with the cell nuclei, consistent with previous reports and referred to as foci, more general  Phosphorylated H2AX (γ-H2AX) is essential to the efficient recognition and (or) repair of DNA double strand breaks (DSBs), and many molecules, often  The formation of γ-H2AX foci has also been shown after replication stalling and single-stranded DNA breaks (24, 39, 55). In that case, the phosphorylation of H2AX  Aug 1, 2010 A marker for DSB formation is the phosphorylated form of the histone H2 variant H2AX (γ-H2AX). DSBs also attract the damage sensor  The histone variant H2AX is rapidly phosphorylated at the sites of DNA double- strand breaks (DSBs).

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H2A histone family member X (usually abbreviated as H2AX) is a type of histone protein from the H2A family encoded by the H2AFX gene. An important phosphorylated form is γH2AX (140S), which forms when double-strand breaks appear. The H2AX histone was first reported by West and Bonner in 1980, as a specific variant of the H2A histone family. H2AX is ubiquitously distributed along the genome and differs from the other members of the family by the presence of an evolutionary conserved C-terminal motif: KKATQASQEY (Rogakou et al., 2000; Takahashi and Ohnishi, 2005). Detection of human gamma-H2AX by western blot. Samples: Whole cell lysate (15 µg) from Jurkat cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-).

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In the  4 Feb 2010 Phosphorylation of the Ser-139 residue of the histone variant H2AX, forming γH2AX, is an early cellular response to the induction of DNA  20 Sep 2011 We propose here to quantify γ-H2AX in CTCs as a measurement of DNA damage in the tumor in patients receiving veliparib (ABT-888), an oral  20 Jun 2007 10565 Background: DNA double-strand breaks (DSBs) caused by exposure to DNA damaging agents initiate phosphorylation of histone H2AX  The gamma-H2AX assay enables screening for functional integrity of DNA double strand break (DSB) repair, and a broad range of genetic and epigenetic  gamma-H2AX Antibody. Gene and Protein Information. H2AX is a member of the histone H2A family.

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Gamma-H2AX is involved in DNA double-strand break repair through both the non-homologous end-joining and homologous recombination. These results unveil a new interplay between plant H2AX and E2F transcriptional activators during the DNA double-strand breaks response. Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). Phospho-H2AX or γ-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. The results suggest gamma-H2AX is a useful adjunct in diagnosis of metastatic RCC when RCC-Ma is negative and in higher grade RCC, which are often a diagnostic challenge. A nuclear pattern of staining of gamma-H2AX has a comparable sensitivity with RCC-Ma, and the interpretation is easier and more reliable.

Show more  ground state, accompanied by gamma radiation of 1460 keV energy in gamma-H2AX foci in cells co-cultured with irradiated ones, or treated with ICM only,  The number distribution of gamma photons F (n) generated in . An optimized method for detecting gamma-H2AX in blood cells . thermal neutrons, gamma-rays. K ey detector mma-H2AX assay, blood simplified scheme of reactions and corresponding gamma rays co ming from. (inaktivt). 2008-12-17, Studier av gamma-sekretas, ett proteinkomplex involverat i 2008-01-17, H2AX-metoden för mätning av DNA-skador efter exponering för  71 Trimera G-protein består av subenheterna alfa, beta och gamma.
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In the detection of genomic damage, gamma-H2AX is especially important in research involving cancer treatment and … Compare gammaH2ax ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. Laboratories around the world now use the gamma-H2AX assay developed by Bonner to study how cells sense and respond to double-strand breaks. The CCR team also used the gamma-H2AX assay to discover that cells’ genomic integrity may decline as we age in part because aging cells assemble damage repair machinery more slowly.

A gamma-H2AX kit and antibody allows the assessment of DNA damage and DNA repair for ELISA based assays, immunohistochemistry or flow cytometry. In the detection of genomic damage, gamma-H2AX is especially important in research involving cancer treatment and … Laboratories around the world now use the gamma-H2AX assay developed by Bonner to study how cells sense and respond to double-strand breaks. The CCR team also used the gamma-H2AX assay to discover that cells’ genomic integrity may decline as we age in part because aging cells assemble damage repair machinery more slowly. Keywords: Gamma-H2AX, High-content screening, Image processing, Hough transform, dosimetry 1. INTRODUCTION The study of damage to cellular DNA is essential for the understanding of cell apoptosis and mutation that may lead to serious disease including cancer. Changes in the genome caused by a single double strand break (DSB) may be enough Phosphorylated on Ser-140 (to form gamma-H2AX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Gamma H2AX foci are always formed in case of DNA double strand breaks as a first step in its repair.
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Detection of human gamma-H2AX by western blot. Samples: Whole cell lysate (15 µg) from Jurkat cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibody: Affinity purified rabbit anti-gamma-H2AX antibody A300-081A (lot A300-081A-19) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Se hela listan på academic.oup.com 2017-02-03 · Phosphorylated H2AX (γ-H2AX) is a sensitive marker for DNA double-strand breaks (DSBs), but the variability of H2AX expression in different cell and tissue types makes it difficult to interpret the meaning of the γ-H2AX level. Furthermore, the assays commonly used for γ-H2AX detection utilize laborious and low-throughput microscopy-based methods. We describe here an ELISA assay that Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX.
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Using gammaH2AX detection to determine the extent of DSB induction may help to detect precancerous cells, Gamma-H2AX can also be measured by immunohistochemistry. By counting gamma-H2AX- foci, it is possible to measure very low levels of DNA damage that are inflicted during routine X-ray examinations . A comparison of our flow-cytometry method with published data indicates that foci counting can detect close to ten times lower doses of IR . Anti-Gamma H2AX (phospho-Ser139) antibody, Rabbit monoclonal recombinant, expressed in HEK 293 cells, clone RM224, purified immunoglobulin Synonym: H2AX Ser139 p, Histone H2AX (phospho ser139) Keywords: Gamma-H2AX, High-content screening, Image processing, Hough transform, dosimetry 1. INTRODUCTION The study of damage to cellular DNA is essential for the understanding of cell apoptosis and mutation that may lead to serious disease including cancer. Changes in the genome caused by a single double strand break (DSB) may be enough Compare gammaH2ax ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.

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Phosphorylated on Ser-140 (to form gamma-H2AX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb in the presence of control peptide (left) or Phospho-Histone H2A.X (Ser139) Blocking Peptide #1260 (right). Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. 2016-03-31 · Rabbit polyclonal gamma H2A.X (phospho S139) antibody. Validated in WB, ICC/IF and tested in Human. Cited in 269 publication(s). Independently reviewed in 35 review(s).

gamma-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double-strand break repair. Chowdhury D, Keogh MC, Ishii H, Peterson CL, Buratowski S, Lieberman J. Mol Cell. 2005 Dec 9;20(5):801-9. 2010-11-23 Gamma-H2AX is lost from chromatin surrounding a double-strand break independently of the HTP-C, indicating that the phosphatase targets gamma-H2AX after its displacement from DNA. The dephosphorylation of gamma-H2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint. Gamma-H2AX foci in cells exposed to a mixed beam of X-rays and alpha particles Elina Staaf1*, Karl Brehwens1, Siamak Haghdoost1, Joanna Czub2 and Andrzej Wojcik1,3 Abstract Background: Little is known about the cellular effects of exposure to mixed beams of … Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody, Histone Gamma-H2A.X (Phospho S139) Clone RM224 quantity Add to cart SKU: 31-1104-00 Categories: Histone H2A.Z Acetylation , Histone Modification , Primary Antibodies , Rabbit Monoclonal Tags: 31-1104-00 , 31-1104-00-S , 31110400 , 31110400S Compare Anti-gammaH2ax Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. 2021-02-25 Phosphorylated H2AX (gamma-H2AX) is essential to the efficient recognition and (or) repair of DNA double strand breaks (DSBs), and many molecules, often thousands, of H2AX become rapidly phosphorylated at the site of each nascent DSB. An antibody to gamma-H2AX reveals that this highly amplified process generates nuclear foci.